Article
SILIP: a novel stable isotope labeling method for in planta quantitative proteomic analysis.
The Plant journal : for cell and molecular biology - 1 Dec 2008
Schaff Jennifer E, Mbeunkui Flaubert, Blackburn Kevin, Bird David McK, Goshe Michael B
Abstract excerpt
Due to ease of manipulation, metabolic isotope coding of samples for proteomic analysis is typically performed in cell culture, thus preventing an accurate in vivo quantitative analysis, which is only achievable in intact organisms. To address this issue in plant biology, we developed SILIP (stable isotope labeling in planta) using tomato plants (Solanum lycopersicum cv. Rutgers) as a method that allows...
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