Article
Sequence and expression in Escherichia coli of a Mycoplasma hominis gene encoding elongation factor Tu.
Gene - 15 Jun 1991
Lüneberg E, Kamla V, Hadding U, Frosch M
Abstract excerpt
We describe the molecular cloning and the complete nucleotide (nt) sequence of a Mycoplasma hominis gene common to a broad range of Mycoplasma species, as defined by hybridization analysis with the cloned gene. Production of M. hominis protein in Escherichia coli was assayed by use of a monoclona...
Topics
- Amino Acid Sequence
- Antibodies, Monoclonal
- Antigens, Bacterial
- Base Sequence
- Blotting, Southern
- Blotting, Western
- Cloning, Molecular
- Escherichia coli
- Genetic Variation
- Molecular Sequence Data
- Multigene Family
- Mycoplasma
- Peptide Elongation Factor Tu
- Plasmids
- Recombinant Proteins
- Restriction Mapping
- Sequence Homology, Nucleic Acid