Article
Cloning, sequencing and variability analysis of the gap gene from Mycoplasma hominis.
FEMS microbiology letters - 1 Feb 2000
Mygind T, Zeuthen Søgaard I, Melkova R, Boesen T, Birkelund S, Christiansen G
Abstract excerpt
The gap gene encodes the glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The gene was cloned and sequenced from the Mycoplasma hominis type strain PG21(T). The intraspecies variability was investigated by inspection of restriction fragment length polymorphism (RFLP) patterns after polymerase chain reaction (PCR) amplification of the gap gene from 15 strains and furthermore by sequencing of...
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