Article
Arginine coordination in enzymatic phosphoryl transfer: evaluation of the effect of Arg166 mutations in Escherichia coli alkaline phosphatase.
Biochemistry - 22 Jul 2008
O'Brien Patrick J, Lassila Jonathan Kyle, Fenn Timothy D, Zalatan Jesse G, Herschlag Daniel
Abstract excerpt
Arginine residues are commonly found in the active sites of enzymes catalyzing phosphoryl transfer reactions. Numerous site-directed mutagenesis experiments establish the importance of these residues for efficient catalysis, but their role in catalysis is not clear. To examine the role of arginine residues in the phosphoryl transfer reaction, we have measured the consequences of mutations to arginine 166 in...
Topics
- Alkaline Phosphatase
- Arginine
- Escherichia coli
- Escherichia coli Proteins
- Hydrolysis
- Mutagenesis, Site-Directed
- Mutation
- Phosphates
- Structure-Activity Relationship
- Substrate Specificity
- X-Ray Diffraction