Article
Phenotype differentiation of three E. coli strains by GC-FID and GC-MS based metabolomics.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences - 15 Aug 2008
Tian Jing, Shi Chunyun, Gao Peng, Yuan Kailong, Yang Dawei, Lu Xin, Xu Guowang
Abstract excerpt
Two mutants of E. coli with deletion of sdhAB and ackA-pta genes respectively and their wild-type strains were subjected to gas chromatography-flame-ionization detection (GC-FID) and gas chromatography-mass spectrometry (GC-MS) metabolomics analysis. Intracellular metabolites of the three strains were profiled by GC-FID firstly. Methodological evaluation of the employed platform indicated that the limit of...
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