Article
PCR-SSCP-DNA sequencing method in detecting PTEN gene mutation and its significance in human gastric cancer.
World journal of gastroenterology - 28 Jun 2008
Guo Chuan-Yong, Xu Xuan-Fu, Wu Jian-Ye, Liu Shu-Fang
Abstract excerpt
AIM: To discuss the possible effect of PTEN gene mutations on occurrence and development of gastric cancer. METHODS: Fifty-three gastric cancer specimens were selected to probe PTEN gene mutations in genome of gastric cancer and paracancerous tissues using PCR-SSCP-DNA sequencing method based on microdissection and to observe the protein expression by immunohistochemistry technique. RESULTS: PCR-SSCP-DNA...
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