Article
Work flow for multiplexing siRNA assays by solid-phase reverse transfection in multiwell plates.
Journal of biomolecular screening - 1 Aug 2008
Erfle Holger, Neumann Beate, Rogers Phill, Bulkescher Jutta, Ellenberg Jan, Pepperkok Rainer
Abstract excerpt
Solid-phase reverse transfection on cell microarrays is a high-throughput method for the parallel transfection of mammalian cells. However, the cells transfected in this way have been restricted so far to microscopy-based analyses. Analysis methods such as reverse transcriptase-polymerase chain reaction (RT-PCR) and access to higher cell numbers for statistical reasons in microscopy-based assays are not possible...
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