Article
Mutations in transhydrogenase change the fluorescence emission state of TRP72 from 1La to 1Lb.
Biophysical journal - 1 Oct 2008
Tveen Jensen Karina, Strambini Giovanni, Gonnelli Margherita, Broos Jaap, Jackson J Baz
Abstract excerpt
The dI component of Rhodospirillum rubrum transhydrogenase has a single Trp residue (Trp(72)), which has distinctive optical properties, including short-wavelength fluorescence emission with clear vibrational fine structure, and long-lived, well-resolved phosphorescence emission. We have made a set of mutant dI proteins in which residues contacting Trp(72) are conservatively substituted. The room-temperature...
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