Article
Triple mutation of Cys26, Trp35, and Cys126 in stromal ascorbate peroxidase confers H2O2 tolerance comparable to that of the cytosolic isoform.
Biochemical and biophysical research communications - 8 Aug 2008
Kitajima Sakihito, Kitamura Masahito, Koja Namiko
Abstract excerpt
Ascorbate peroxidase (APX) isoforms localized in the stroma and thylakoid of the chloroplast play a principle role in detoxifying hydrogen peroxide (H(2)O(2)) generated in photosystem I; however, once the ascorbate is depleted, the enzyme is attacked by H(2)O(2) and rapidly loses its activity. Here, we report that radical transfer across the porphyrin moiety and amino acid residues in the reaction intermediate...
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