Article
Evidence that the amino acid residue Cys117 of chloroplastic monodehydroascorbate reductase is involved in its activity and structural stability.
International journal of biological macromolecules - 1 Apr 2010
Li Feng, Wu Qing-Yun, Sun Yan-Li, Ma Na-Na, Wang Xiao-Yun, Meng Qing-Wei
Abstract excerpt
Monodehydroascorbate reductase (MDAR; EC 1.6.5.4) is crucial for AsA regeneration and essential for maintaining the reduced pool of AsA. And the amino acid residue C117 of chloroplastic MDAR is the conserved cysteine residue in MDAR isoforms. A series mutation of conserved amino acid residue cysteine117 (C117) was constructed to investigate its role in MDAR structural stability and activity. Our study revealed...
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