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Article

Simultaneous detection and typing of genital human papillomavirus DNA using the polymerase chain reaction

1 May 1991

Abstract excerpt

A simple method has been developed for detecting a broad range of genital human papillomavirus (HPV) types using the polymerase chain reaction (PCR). We utilized two consensus sequence primer pairs within the E6 and E7 open reading frames to amplify HPV DNA; malignant HPV DNA (from HPV-16, -18, -31, -33, -52b and -58) was amplified using the pU-1M/pU-2R primer pair whereas benign HPV DNA (from HPV-6 and -11) was...

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PMID: 1851805
DOI: 10.1099/0022-1317-72-5-1039

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