Article
Differential detection of human papillomavirus DNA type 6 and 11 amplified by polymerase chain reaction.
Journal of dermatological science - 1 Sept 1990
Liew F M, Yamanishi K, Kishimoto S, Yasuno H
Abstract excerpt
We have developed a method for differential detection of human papillomavirus type 6 and 11 with selective amplification of a segment of the viral DNAs by the polymerase chain reaction. The target sequence for amplification was a 134-bp (bp 206-339) in the E6 open reading frame of HPV 6 and 11 DNAs. DNA extracted from a paraffin-section or a minute fresh biopsy specimen of condyloma acuminatum was amplified and...
Topics
- Base Sequence
- Condylomata Acuminata
- DNA, Viral
- Diagnosis, Differential
- Gene Amplification
- Genotype
- Humans
- Male
- Molecular Sequence Data
- Oligonucleotide Probes
- Papillomaviridae
