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Article

Megaplasmid pRme2011a of Sinorhizobium meliloti is not required for viability.

Journal of bacteriology - 1 Jun 2000

Oresnik I J, Liu S L, Yost C K, Hynes M F

Abstract excerpt

We report the curing of the 1,360-kb megaplasmid pRme2011a from Sinorhizobium meliloti strain Rm2011. With a positive selection strategy that utilized Tn5B12-S containing the sacB gene, we were able to cure this replicon by successive rounds of selecting for deletion formation in vivo. Subsequent Southern blot, Eckhardt gel, and pulsed-field gel electrophoresis analyses were consistent with the hypothesis that...

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Identifiers

PMID: 10852892
DOI: 10.1128/JB.182.12.3582-3586.2000
PMCID: PMC101967

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Source receipt: pubmed, retrieved 7 Sept 2026.

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