Article
Molecular cloning of a cDNA of a camptothecin-resistant human DNA topoisomerase I and identification of mutation sites.
Nucleic acids research - 11 Jan 1991
Tamura H, Kohchi C, Yamada R, Ikeda T, Koiwai O, Patterson E, Keene J D, Okada K, Kjeldsen E, Nishikawa K
Abstract excerpt
Camptothecin (CPT), a plant alkaloid with antitumor activity, is a specific inhibitor of eukaryotic DNA topoisomerase I. We have previously isolated and characterized a CPT-resistant topoisomerase I isolated from a CPT-resistant human leukemia cell line, CPT-K5. cDNA clones of topoisomerase I wer...
Topics
- Amino Acid Sequence
- Blotting, Southern
- Camptothecin
- Cloning, Molecular
- DNA
- DNA Topoisomerases, Type I
- Drug Resistance
- Escherichia coli
- Gene Expression
- Humans
- Molecular Sequence Data
- Mutation
- Protein Conformation
- Recombinant Fusion Proteins