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Article

Molecular cloning of a cDNA of a camptothecin-resistant human DNA topoisomerase I and identification of mutation sites.

Nucleic acids research - 11 Jan 1991

Tamura H, Kohchi C, Yamada R, Ikeda T, Koiwai O, Patterson E, Keene J D, Okada K, Kjeldsen E, Nishikawa K

Abstract excerpt

Camptothecin (CPT), a plant alkaloid with antitumor activity, is a specific inhibitor of eukaryotic DNA topoisomerase I. We have previously isolated and characterized a CPT-resistant topoisomerase I isolated from a CPT-resistant human leukemia cell line, CPT-K5. cDNA clones of topoisomerase I were isolated from the CPT-resistant and the parental CPT-sensitive cell lines, respectively. Sequencing of the clones...

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Identifiers

PMID: 1849260
DOI: 10.1093/nar/19.1.69
PMCID: PMC333535

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