Article
Functional analysis of the MATB mating-type idiomorph of the dimorphic fungus Yarrowia lipolytica.
Current microbiology - 1 Aug 2008
Rosas-Quijano Raymundo, Gaillardin Claude, Ruiz-Herrera José
Abstract excerpt
The whole MATA cassette from Yarrowia lipolytica, a dimorphic fungus, was replaced by the URA3 gene through a double homologous recombination. This MAT-less strain lost its mate capacity with A or B Y. lipolytica strains. Introduction of polymerase chain reaction-synthesized idiomorph MATB in a null strain of A locus by double homologous recombination gave rise to a "transsexual" B strain. Mating capacity of this...
Topics
- DNA, Fungal
- Gene Deletion
- Genes, Mating Type, Fungal
- Genotype
- Phenotype
- Recombination, Genetic
- Yarrowia