Article
Isolation of embryo-specific mutants in Arabidopsis: genetic and phenotypic analysis.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2008
Liu Nai-You, Zhang Zhi-Feng, Yang Wei-Cai
Abstract excerpt
To perform an effective genetic screen for embryo-specific mutants is a prerequisite for understanding the molecular mechanisms that control Arabidopsis embryo development. Mutagenesis based on either T-DNA or transposon insertion has been successfully used in identifying embryonic mutants. We present here a typical genetic screen for putative embryonic mutants based on distorted Mendelian segregation ratio (2:1)...
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