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Article

Insertional mutagenesis of genes required for seed development in Arabidopsis thaliana.

Genetics - 1 Dec 2001

McElver J, Tzafrir I, Aux G, Rogers R, Ashby C, Smith K, Thomas C, Schetter A, Zhou Q, Cushman M A, Tossberg J, Nickle T, Levin J Z, Law M, Meinke D, Patton D

Abstract excerpt

The purpose of this project was to identify large numbers of Arabidopsis genes with essential functions during seed development. More than 120,000 T-DNA insertion lines were generated following Agrobacterium-mediated transformation. Transgenic plants were screened for defective seeds and putative mutants were subjected to detailed analysis in subsequent generations. Plasmid rescue and TAIL-PCR were used to...

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Identifiers

PMID: 11779812
DOI: 10.1093/genetics/159.4.1751
PMCID: PMC1461914

Genes

EMB

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Source receipt: pubmed, retrieved 7 Sept 2026.

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