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Mimicking phosphorylation of Ser‐74 on human deoxycytidine kinase selectively increases catalytic activity for dC and dC analogues

5 Feb 2008

Abstract excerpt

Intracellular phosphorylation of dCK on Ser-74 results in increased nucleoside kinase activity. We mimicked this phosphorylation by a Ser-74-Glu mutation in bacterially produced dCK and investigated kinetic parameters using various nucleoside substrates. The S74E mutation increases the k(cat) values 11-fold for dC, and 3-fold for the anti-cancer analogues dFdC and AraC. In contrast, the rate is decreased for the...

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PMID: 18258203
DOI: 10.1016/j.febslet.2008.01.048

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