Article
Mechanism of copper induced fluorescence quenching of red fluorescent protein, DsRed.
Biochemical and biophysical research communications - 23 May 2008
Rahimi Yasmeen, Goulding Ann, Shrestha Suresh, Mirpuri Sweetie, Deo Sapna K
Abstract excerpt
The red fluorescent protein, DsRed, and a few of its mutants have been shown to bind copper ions resulting in quenching of its fluorescence. The response to Cu(2+) is rapid, selective, and reversible upon addition of a copper chelator. DsRed has been employed as an in vitro probe for Cu(2+) determination by us and other groups. It is also envisioned that DsRed can serve as an intracellular genetically encoded...
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