Article
Secretion pathway for the poly(3-hydroxybutyrate) depolymerase in Ralstonia pickettii T1.
Antonie van Leeuwenhoek - 1 Aug 2008
Sugimoto Akiko, Shiraki Mari, Hatakeyama Sachie, Saito Terumi
Abstract excerpt
The extracellular poly(3-hydroxybutyrate) depolymerase from Ralstonia pickettii T1 has been purified, its function and character investigated in detail, and its gene cloned and sequenced. However, the mechanism by which this enzyme is secreted has not been elucidated. A mutant unable to degrade poly(3-hydroxybutyrate), N17, was obtained with the random insertion of a mini-transposon, Tn5. Western analysis using...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
