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Impact of a random TN5 mutation on endoglucanase secretion in ruminal cellulolytic Escherichia coli.

Gene - 15 Jan 2025

Pang Jian, Zhang Long, Qi Qingsheng, Liu Zhanying

Abstract excerpt

OBJECTIVE: Most protein secretion systems are found in gram-negative bacteria, but the mechanism of endoglucanase (BcsZ) secretion in Escherichia coli (E. coli) remains unclear. METHODS: In this study, we used JBZ-DH5α (which overexpresses BcsZ on the E. coli DH5α genome) as the initial strain. A mutant library was created by randomly inserting the TN5 transposon into the genome, and mutants with reduced...

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PMID: 39260626
DOI: 10.1016/j.gene.2024.148936

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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