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Effects of mutations in the substrate-binding domain of poly[(R)-3-hydroxybutyrate] (PHB) depolymerase from Ralstonia pickettii T1 on PHB degradation.

Applied and environmental microbiology - 1 Nov 2006

Hiraishi Tomohiro, Hirahara Yoko, Doi Yoshiharu, Maeda Mizuo, Taguchi Seiichi

Abstract excerpt

Poly[(R)-3-hydroxybutyrate] (PHB) depolymerase from Ralstonia pickettii T1 (PhaZ(RpiT1)) adsorbs to denatured PHB (dPHB) via its substrate-binding domain (SBD) to enhance dPHB degradation. To evaluate the amino acid residues participating in dPHB adsorption, PhaZ(RpiT1) was subjected to a high-throughput screening system consisting of PCR-mediated random mutagenesis targeted to the SBD gene and a plate assay to...

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PMID: 16963553
DOI: 10.1128/AEM.01187-06
PMCID: PMC1636158

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