Article
Determination of the active sites serine of the poly (3-hydroxybutyrate) depolymerases of Pseudomonas lemoignei (PhaZ5) and of Alcaligenes faecalis.
FEMS microbiology letters - 15 Jul 1996
Shinohe T, Nojiri M, Saito T, Stanislawski T, Jendrossek D
Abstract excerpt
Mutational analysis of the poly(3-hydroxybutyrate) (PHB) depolymerase A of Pseudomonas lemoignei and of the poly(3-hydroxybutyrate) depolymerase of Alcaligenes faecalis revealed that S138 (P. lemoignei) and S139 (A. faecalis) are essential for activity. Both serines are part of a strictly conserv...
Topics
- Alcaligenes
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- Carboxylic Ester Hydrolases
- Enzyme Inhibitors
- Escherichia coli
- Isoflurophate
- Molecular Sequence Data
- Molecular Weight
- Mutation
- Pseudomonas
- Recombinant Fusion Proteins
- Sequence Alignment
- Serine