Article
Expression of recombinant human plasminogen in mammalian cells is augmented by suppression of plasmin activity.
The Journal of biological chemistry - 15 Aug 1991
Busby S J, Mulvihill E, Rao D, Kumar A A, Lioubin P, Heipel M, Sprecher C, Halfpap L, Prunkard D, Gambee J
Abstract excerpt
We present evidence that over-expression of human plasminogen, the precursor to the serine protease plasmin, can be cytotoxic to mammalian cells. When an expression vector containing plasminogen cDNA is transfected into baby hamster kidney cells, the number of drug-resistant colonies as well as t...
Topics
- Animals
- Autoradiography
- Base Sequence
- Cell Survival
- Cells, Cultured
- Cricetinae
- DNA
- Fibrinolysin
- Humans
- Molecular Sequence Data
- Mutation
- Plasmids
- Plasminogen
- Precipitin Tests
- RNA, Antisense
- Recombinant Proteins
- Transfection
- Urokinase-Type Plasminogen Activator