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Article

Quantitative Single-letter Sequencing: a method for simultaneously monitoring numerous known allelic variants in single DNA samples.

BMC genomics - 21 Feb 2008

Monsion Baptiste, Duborjal Hervé, Blanc Stéphane

Abstract excerpt

BACKGROUND: Pathogens such as fungi, bacteria and especially viruses, are highly variable even within an individual host, intensifying the difficulty of distinguishing and accurately quantifying numerous allelic variants co-existing in a single nucleic acid sample. The majority of currently available techniques are based on real-time PCR or primer extension and often require multiplexing adjustments that impose a...

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PMID: 18291029
DOI: 10.1186/1471-2164-9-85
PMCID: PMC2276495

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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