Reversible male sterility in transgenic tobacco carrying a dominant-negative mutated glutamine synthetase gene under the control of microspore-specific promoter.

Abstract excerpt

Metabolic engineering was used to disrupt glutamine metabolism in microspores in order to block pollen development. We used a dominant-negative mutant (DNM) approach of cytosolic glutamine synthetase (GS1) gene under the microspore-specific promoter NTM19 to block glutamine synthesis in developing pollen grains. We observed partial male sterility in primary transgenic plants by using light microscopy, FDA, DAPI...

Topics

• Amino Acids
• Genes, Dominant
• Glutamate-Ammonia Ligase
• Glutamine
• Mutation
• Plant Infertility
• Plants, Genetically Modified
• Pollen
• Promoter Regions, Genetic
• Nicotiana