Article
Modulation of glutamine synthetase gene expression in tobacco by the introduction of an alfalfa glutamine synthetase gene in sense and antisense orientation: molecular and biochemical analysis.
Molecular & general genetics : MGG - 1 Jan 1993
Temple S J, Knight T J, Unkefer P J, Sengupta-Gopalan C
Abstract excerpt
A glutamine synthetase (GS) cDNA isolated from an alfalfa cell culture cDNA library was found to represent a cytoplasmic GS. The full-length alfalfa GS1 coding sequence, in both sense and antisense orientation and under the transcriptional control of the cauliflower mosaic virus 35S promoter, was introduced into tobacco. Leaves of tobacco plants transformed with the sense construct contained greatly elevated...
Topics
- Alcohol Oxidoreductases
- Cells, Cultured
- Cloning, Molecular
- Down-Regulation
- Gene Expression
- Genetic Variation
- Glutamate-Ammonia Ligase
- Hydroxypyruvate Reductase
- Medicago sativa
- Nucleic Acid Hybridization