Article
[Construction of hyaluronidase gene mutant of Enterococcus faecium and the function of hyaluronidase gene].
Zhonghua yi xue za zhi - 30 Oct 2007
Wu Li-xian, Huang Wen-xiang, Luo Tao, Sun Xiao-ping
Abstract excerpt
OBJECTIVE: To construct a hyaluronidase (hyl) gene mutant strain of Enterococcus faecium and to study the function of hyl gene. METHODS: An isogenic hyl-deficient mutant (*hyl) with suicide plasmid pTX4577 was constructed and screened by allelic replacement. The mutant strain and wild type strain TX2466 were cultured to observe the growth curves. Both *hyl and TX2466 strains were cultured in solid with kanamycin...
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