Article
[Study on pathogenicity of putative virulence gene of Enterococcus faecium].
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi - 1 Jun 2009
Wu Lixian, Huang Wenxiang, Wang Guofu, Sun Xiaoping
Abstract excerpt
The presence of hyl gene in 364 PFGE clones of Enterococcus faecium was detected by colony hybridization under conditions of high stringency. The isogenic hyl-deficient mutant (* hyl) was constructed with suicide pTX4577 and screened by allelic replacement. Moreover, an in vitro study was made on the effect of hyl gene detection on the growth ability of hylgene detection on the mutant, and an in vivo study was...
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