Article
Repopulating activity of ex vivo-expanded murine hematopoietic stem cells resides in the CD48-c-Kit+Sca-1+lineage marker- cell population.
Stem cells (Dayton, Ohio) - 1 Mar 2008
Noda Shinichi, Horiguchi Kana, Ichikawa Hitoshi, Miyoshi Hiroyuki
Abstract excerpt
A better understanding of the biology of cultured hematopoietic stem cells (HSCs) is required to achieve ex vivo expansion of HSCs. In this study, clonal analysis of the surface phenotype and repopulating activity of ex vivo-expanded murine HSCs was performed. After 7 days of culture with stem cell factor, thrombopoietin, fibroblast growth factor-1, and insulin-like growth factor-2, single...
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