Article
Tn5 insertion in the tonB gene promoter affects iron-related phenotypes and increases extracellular siderophore levels in Gluconacetobacter diazotrophicus.
Archives of microbiology - 1 Mar 2015
de Paula Soares Cleiton, Rodrigues Elisete Pains, de Paula Ferreira Jéssica, Simões Araújo Jean Luiz, Rouws Luc Felicianus Marie, Baldani José Ivo, Vidal Marcia Soares
Abstract excerpt
TonB-dependent receptors in concert with the TonB-ExbB-ExbD protein complex are responsible for the uptake of iron and substances such as vitamin B12 in several bacterial species. In this study, Tn5 mutagenesis of the sugarcane endophytic bacterium Gluconacetobacter diazotrophicus led to the isolation of a mutant with a single Tn5-insertion in the promoter region of a tonB gene ortholog. This mutant, named...
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