Article
A practical and cost-effective mutation scanning-based approach for investigating genetic variation in Cryptosporidium.
Electrophoresis - 1 Nov 2007
Jex Aaron R, Whipp Margaret, Campbell Bronwyn E, Cacciò Simone M, Stevens Melita, Hogg Geoff, Gasser Robin B
Abstract excerpt
In the present study, we used a mutation scanning-targeted sequencing approach to assess variation in part (pgp60) of the 60 kDa glycoprotein (gp60) gene among Cryptosporidium samples from humans in Victoria, Australia. Two nuclear ribosomal loci (the small subunit rRNA gene and the second internal transcribed spacer) were used to identify the samples as Cryptosporidium hominis (n = 74), Cryptosporidium parvum (n...
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