Article
Isolation and characterization of corneal endothelial cells from wild type and thrombospondin-1 deficient mice.
Molecular vision - 27 Aug 2007
Scheef Elizabeth A, Huang Qiong, Wang Shoujian, Sorenson Christine M, Sheibani Nader
Abstract excerpt
PURPOSE: To isolate and characterize primary corneal endothelial cells (CEC) from wild type and transgenic mice to facilitate the study of their properties in vitro. METHODS: CEC were isolated from wild type or transgenic-immortomice corneas. The Descemet's membrane was gently peeled from the periphery of the cornea towards the central region and placed into wells of a 96 well tissue culture plate coated with...
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