Article
[Cloning and detailed mapping of the fra-ymt region of the Yersinia pestis pFra plasmid].
Molekuliarnaia genetika, mikrobiologiia i virusologiia - 1 Dec 1991
Cherepanov P A, Mikhaĭlova T G, Karimova G A, Zakharova N M, Ershov Iu V, Volkovoĭ K I
Abstract excerpt
The genetical libraries of the pFra plasmid of Yersinia pestis genes were obtained by insertion into the PstI, SalGI, EcoRI, XhoI restriction sites of the cosmid vector pHC79. The immunochemical analysis of the recombinant clones has revealed the clones synthesizing the antigen Fl (fraction I) and mouse toxin (Ymt--Yersinia pestis murine toxin). The restriction analysis of the plasmids from antigen synthesizing...
Topics
- Amino Acid Sequence
- Antigens, Bacterial
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Genes, Bacterial
- Immunohistochemistry
- Molecular Sequence Data
