Article
[Specific proteolysis by fibrinolysin-coagulase from Yersinia pestis of Yersinia pseudotuberculosis outer membrane proteins coded by the Ca(2+)-dependence plasmid].
Molekuliarnaia genetika, mikrobiologiia i virusologiia - 1 Jan 1991
Shmelev V A, Cherepanov P A, Nosova L Iu, Karimova G A, Popov S G, Noskov A N
Abstract excerpt
The pesticinogenicity 9.5 kb plasmid from Yersinia pestis strain EV76 has been marked by the kanamycin phosphotransferase gene inserted into PstI site and designated pP3. The obtained plasmid pP3 determines the synthesis of 45 kd pesticin, alpha and beta-forms of fibrinolysin coagulase (37 and 35 kd) and the 29, 19 and 13 kd proteins in Escherichia coli mini cells. When transferred into Yersinia...
Topics
- Autoradiography
- Bacterial Outer Membrane Proteins
- Calcium
- Coagulase
- Electrophoresis, Polyacrylamide Gel
- Fibrinolysin
- Genes, Bacterial
- Hydrolysis
- Mutation
- Plasmids