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Article

Effects of the size and number of zona pellucida openings on hatching and trophoblast outgrowth in the mouse embryo

1 Sept 1991

Abstract excerpt

The present studies were performed to establish the effects of the size and number of artificial holes produced in the zona pellucida (ZP) on hatching and trophoblast outgrowth in vitro. Limited partial zona dissection (PZD) produced small, narrow incisions, and zona drilling with acidic Tyrode's (AT) across a larger area in the ZP was used to produce bigger round holes. Some embryos were micromanipulated once;...

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PMID: 1781990
DOI: 10.1002/mrd.1080300110

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