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Chemically Assisted Handmade Enucleation of Porcine Oocytes

1 Dec 2006

Abstract excerpt

The purpose of our work was to find an efficient and reliable chemically assisted procedure for enucleation of porcine oocytes connected to the handmade cloning (HMC) technique without the potentially harmful chromatin staining and ultraviolet (UV) irradiation for cytoplast selection. After 41-42 h in vitro maturation, porcine oocytes were incubated with 0.4 microg/mL demecolcine for 45 min. Subsequently, the...

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PMID: 17196089
DOI: 10.1089/clo.2006.8.241

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