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Developmental Ability of Zona-Free Mouse Embryos is Influenced by Cell Association at the 4-Cell Stage

1 Jul 1995

Abstract excerpt

Zona-free embryos at the 4-cell stage were classified into four types according to the total points of contact between the 4 blastomeres. Types A, B, C, and D had 3, 4, 5, and 6 points of contact, respectively. Embryos of these types were cultured individually in 20 microliters of Whitten's medium. Blastocysts derived from types A and D embryos at the 4-cell stage were transferred and observed on Day 17 of...

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PMID: 7669859
DOI: 10.1095/biolreprod53.1.78

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