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Article

Somatic Cell Cloning without Micromanipulators

1 Jun 2001

Abstract excerpt

Until now, micromanipulators have been regarded as indispensable for somatic cell nuclear transfer. This paper describes an improved zona-free nuclear transfer procedure with manual bisection of oocytes, selection of cytoplasts by Hoechst staining, and two-step fusion of somatic cells from primary granulosa cell cultures with two cytoplasts. Blastocyst rates in the three systems tested for zona-free embryo...

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PMID: 11900643
DOI: 10.1089/15204550152475590

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