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Use of the Polymerase Chain Reaction for the Specific and Direct Detection of Clostridium dijficile in Human Feces

1 Nov 1991

Abstract excerpt

The polymerase chain reaction was used for the detection of Clostridium difficile, the etiologic agent of antibiotic-associated colitis. An upstream primer identical to a coding region (segment I) of the C. difficile 16S rRNA gene and a downstream primer complementary to a highly conserved region of eubacterial 16S rRNA served to amplify a targeted 270-base-pair fragment of genomic DNA. This technique allowed the...

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PMID: 1775837
DOI: 10.1093/clinids/13.6.1053

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