Article
cis-Acting core RNA elements required for negative-strand RNA synthesis and cap-independent translation are separated in the 3'-untranslated region of Red clover necrotic mosaic virus RNA1.
Virology - 5 Dec 2007
Iwakawa Hiro-Oki, Kaido Masanori, Mise Kazuyuki, Okuno Tetsuro
Abstract excerpt
The genome of Red clover necrotic mosaic virus (RCNMV) is positive-sense and divided into RNA1 and RNA2. RNA1 has a translation enhancer element (3' TE-DR1) in the 3' untranslated region (UTR) that substitutes for a 5' cap. In this study, we determined the regions required for cap-independent translation and RNA synthesis in the 3' UTR of RNA1 using a cell-free extract of evacuolated BY-2 protoplasts (BYL) and by...
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