Article
Direct chemiluminescent imaging detection of human serum proteins in two-dimensional polyacrylamide gel electrophoresis.
Proteomics - 1 Oct 2007
Tao Qian, Wang Zhenzhen, Zhao Heping, Baeyens Willy R G, Delanghe Joris R, Huang Lingyun, Ouyang Jin, He Dacheng, Zhang Xianhua
Abstract excerpt
A novel chemiluminescence (CL)-based imaging method capable of directly detecting proteins in polyacrylamide gels after electrophoresis is proposed. Human serum proteins are presently detected by a direct CL imaging method after native 2-D PAGE. As a consequence, some proteins, including haptoglobin (Hp), Hp precursor, hemopexin (Hpx) precursor, Ig alpha-1 chain C region, and Complement C3 precursor can be...
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