Article
C-terminus engineering of soybean proglycinin: improvement of emulsifying properties.
Protein engineering, design & selection : PEDS - 1 Sept 2007
Prak Krisna, Nakatani Kazuyo, Maruyama Nobuyuki, Utsumi Shigeru
Abstract excerpt
Introduction of the extension region of beta-conglycinin alpha' subunit at the C-terminus of proglycinin A1aB1b results in the improvement of its emulsifying properties. To understand the basic for such improvement, we introduced the alpha' and alpha extension regions to the A2B1a C-terminus, and the alpha extension and A5A4B3 hypervariable regions, and an oligopeptide composed of 20 negatively or positively...
Topics
- Calorimetry, Differential Scanning
- Densitometry
- Dimerization
- Escherichia coli
- Hydrogen-Ion Concentration
- Ions
- Models, Genetic
- Models, Molecular
- Mutation
- Peptides
- Protein Conformation