Article
Ultrahigh-resolution study on Pyrococcus abyssi rubredoxin: II. Introduction of an O-H...Sgamma-Fe hydrogen bond increased the reduction potential by 65 mV.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry - 1 Nov 2007
Bönisch Heiko, Schmidt Christian L, Bianco Pierre, Ladenstein Rudolf
Abstract excerpt
The effect of D-H...S(gamma)-Fe hydrogen bonding on the reduction potential of rubredoxin was investigated by the introduction of an O-H...S(gamma)-Fe hydrogen bond on the surface of Pyrococcus abyssi rubredoxin. The formation of a weak hydrogen bond between Ser44-O(gamma) and Cys42-S(gamma) in mutant W4L/R5S/A44S increased the reduction potential by 56 mV. When side effects of the mutation were taken into...
Topics
- Crystallization
- Crystallography, X-Ray
- Cysteine
- Hydrogen Bonding
- Iron
- Mutation
- Protein Conformation
- Pyrococcus abyssi
- Rubredoxins
- Serine
- Solutions