Article
Structural responses to cavity-creating mutations in an integral membrane protein.
Biochemistry - 18 Sept 2007
Fyfe Paul K, Potter Jane A, Cheng Jade, Williams Christopher M, Watson Ashley J, Jones Michael R
Abstract excerpt
X-ray crystallography has been used to investigate the extent of structural changes in mutants of the purple bacterial reaction center that assemble without a particular ubiquinone or bacteriopheophytin cofactor. In the case of the bacteriopheophytin-exclusion mutant, in which Ala M149 was replaced by Trp (AM149W), the quality of protein crystals was improved over that seen in previous work by minimizing...
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