Article
Regulation of nitrogen fixation in Azorhizobium caulinodans: identification of a fixK-like gene, a positive regulator of nifA.
Molecular microbiology - 1 Aug 1991
Kaminski P A, Mandon K, Arigoni F, Desnoues N, Elmerich C
Abstract excerpt
The nucleotide sequence of a 1 kb fragment upstream of Azorhizobium caulinodans fixL was established. An open reading frame of 744 bp was identified as a fixK homologue. A kanamycin cartridge was inserted into the cloned fixK-like gene and recombined into the host genome. The resulting mutant was Nif-Fix-, suggesting that FixK was required for nitrogen fixation both in symbiotic conditions and in the free-living...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Drug Resistance, Microbial
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Kanamycin
- Kinetics
- Models, Genetic