Article
nifDK Clusters Located on the Chromosome and Megaplasmid of Bradyrhizobium sp. Strain DOA9 Contribute Differently to Nitrogenase Activity During Symbiosis and Free-Living Growth.
Molecular plant-microbe interactions : MPMI - 1 Oct 2016
Wongdee Jenjira, Songwattana Pongpan, Nouwen Nico, Noisangiam Rujirek, Fardoux Joel, Chaintreuil Clémence, Teaumroong Neung, Tittabutr Panlada, Giraud Eric
Abstract excerpt
Bradyrhizobium sp. strain DOA9 contains two copies of the nifDK genes, nifDKc, located on the chromosome, and nifDKp, located on a symbiotic megaplasmid. Unlike most rhizobia, this bacterium displays nitrogenase activity under both free-living and symbiotic conditions. Transcriptional analysis using gusA reporter strains showed that both nifDK operons were highly expressed under symbiosis, whereas nifDKc was the...
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