Article
Azorhizobium caulinodans nitrogen fixation (nif/fix) gene regulation: mutagenesis of the nifA -24/-12 promoter element, characterization of a ntrA(rpoN) gene, and derivation of a model.
Molecular plant-microbe interactions : MPMI - 1 Jan 2000
Stigter J, Schneider M, de Bruijn F J
Abstract excerpt
Using site-directed mutagenesis, mutations were introduced in the -24/-12 promoter element of the Azorhizobium caulinodans nifA gene, and chimeric nifA-lacZ reporter gene fusions were constructed. Single base-pair mutations in the conserved -25 or -13 G residues were found to reduce or abolish ni...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- Conserved Sequence
- DNA Mutational Analysis
- DNA-Binding Proteins
- DNA-Directed RNA Polymerases
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Lac Operon
- Models, Genetic
- Molecular Sequence Data
- Mutagenesis, Insertional