Article
Application of a novel rpoC1-RFLP approach reveals that marine Prochlorococcus populations in the Atlantic gyres are composed of greater microdiversity than previously described.
Microbial ecology - 1 Jan 2008
Jameson Eleanor, Joint Ian, Mann Nicholas H, Mühling Martin
Abstract excerpt
To elucidate the degree of microdiversity within the genus Prochlorococcus, novel Prochlorococcus-specific polymerase chain reaction (PCR) primers were developed for the rpoC1 gene, which encodes the ribonucleic acid (RNA) polymerase core subunit. The size of the PCR fragment (925 bp) coupled with high sequence variation within the rpoC1 fragments (70-99% sequence similarity, 16S ribosomal RNA sequences show...
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