Article
Improved group-specific PCR primers for denaturing gradient gel electrophoresis analysis of the genetic diversity of complex microbial communities.
The ISME journal - 1 Apr 2008
Mühling Martin, Woolven-Allen John, Murrell J Colin, Joint Ian
Abstract excerpt
Phylum- and class-specific PCR primers were tested for the production of clone libraries and for denaturing gradient gel electrophoresis (DGGE) analysis of complex bacterial communities. Primers were designed to specifically amplify 16S rRNA gene fragments of the phyla Bacteroidetes, Planctomycet...
Topics
- Bacteria
- DNA Primers
- DNA, Bacterial
- DNA, Ribosomal
- Ecosystem
- Electrophoresis, Polyacrylamide Gel
- Gene Library
- Genetic Variation
- Molecular Sequence Data
- Phylogeny
- Polymerase Chain Reaction
- RNA, Ribosomal, 16S
- Seawater
- Sequence Analysis, DNA
- Species Specificity